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seh overexpression mice  (Shanghai Model Organisms Center)

 
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    Shanghai Model Organisms Center seh overexpression mice
    Endothelial soluble epoxide hydrolase accelerates atherosclerosis development. A. Representative brightfield image and oil-red-o staining of left carotid artery from wild type and inducible endothelial-specific sEH knockout mice (iΔEC) following 4 weeks of AAV-PCSK9, 4 weeks of HFD and 3 weeks of partial carotid ligation. Quantification of plaque area is shown on the right. Bar = 1 mm and 200 μm in brightfield image and sections, respectively. n = 6 mice/group, two-way ANOVA. B. Representative brightfield image and oil-red-o staining of left carotid artery from wild type and inducible endothelial-specific sEH <t>overexpression</t> mice (iOE) following 4 weeks of AAV-PCSK9, 4 weeks of HFD and 3 weeks of partial carotid ligation. Quantification of plaque area is shown on the right. Bar = 1 mm and 200 μm in brightfield image and sections, respectively. n = 6 mice/group, two-way ANOVA. C. Representative immunefluorescent image of VCAM-1 (green), CD31 (red) and nuclei (DAPI) from left carotid sections of mice as in panel B. Bar = 50 μm. n = 6 mice/group, Student's t-test. D. Representative immunofluorescent image of F4/80 (green), CD31 (red) and nuclei (DAPI) from left carotid sections of mice as in panel B. Bar = 50 μm. n = 6 mice/group, Student's t-test.
    Seh Overexpression Mice, supplied by Shanghai Model Organisms Center, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/seh+overexpression+mice/pmc12214128-163-8-17?v=Shanghai+Model+Organisms+Center
    Average 90 stars, based on 1 article reviews
    seh overexpression mice - by Bioz Stars, 2026-07
    90/100 stars

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    1) Product Images from "Endothelial soluble epoxide hydrolase links polyunsaturated fatty acid metabolism to oxidative stress and atherosclerosis progression"

    Article Title: Endothelial soluble epoxide hydrolase links polyunsaturated fatty acid metabolism to oxidative stress and atherosclerosis progression

    Journal: Redox Biology

    doi: 10.1016/j.redox.2025.103730

    Endothelial soluble epoxide hydrolase accelerates atherosclerosis development. A. Representative brightfield image and oil-red-o staining of left carotid artery from wild type and inducible endothelial-specific sEH knockout mice (iΔEC) following 4 weeks of AAV-PCSK9, 4 weeks of HFD and 3 weeks of partial carotid ligation. Quantification of plaque area is shown on the right. Bar = 1 mm and 200 μm in brightfield image and sections, respectively. n = 6 mice/group, two-way ANOVA. B. Representative brightfield image and oil-red-o staining of left carotid artery from wild type and inducible endothelial-specific sEH overexpression mice (iOE) following 4 weeks of AAV-PCSK9, 4 weeks of HFD and 3 weeks of partial carotid ligation. Quantification of plaque area is shown on the right. Bar = 1 mm and 200 μm in brightfield image and sections, respectively. n = 6 mice/group, two-way ANOVA. C. Representative immunefluorescent image of VCAM-1 (green), CD31 (red) and nuclei (DAPI) from left carotid sections of mice as in panel B. Bar = 50 μm. n = 6 mice/group, Student's t-test. D. Representative immunofluorescent image of F4/80 (green), CD31 (red) and nuclei (DAPI) from left carotid sections of mice as in panel B. Bar = 50 μm. n = 6 mice/group, Student's t-test.
    Figure Legend Snippet: Endothelial soluble epoxide hydrolase accelerates atherosclerosis development. A. Representative brightfield image and oil-red-o staining of left carotid artery from wild type and inducible endothelial-specific sEH knockout mice (iΔEC) following 4 weeks of AAV-PCSK9, 4 weeks of HFD and 3 weeks of partial carotid ligation. Quantification of plaque area is shown on the right. Bar = 1 mm and 200 μm in brightfield image and sections, respectively. n = 6 mice/group, two-way ANOVA. B. Representative brightfield image and oil-red-o staining of left carotid artery from wild type and inducible endothelial-specific sEH overexpression mice (iOE) following 4 weeks of AAV-PCSK9, 4 weeks of HFD and 3 weeks of partial carotid ligation. Quantification of plaque area is shown on the right. Bar = 1 mm and 200 μm in brightfield image and sections, respectively. n = 6 mice/group, two-way ANOVA. C. Representative immunefluorescent image of VCAM-1 (green), CD31 (red) and nuclei (DAPI) from left carotid sections of mice as in panel B. Bar = 50 μm. n = 6 mice/group, Student's t-test. D. Representative immunofluorescent image of F4/80 (green), CD31 (red) and nuclei (DAPI) from left carotid sections of mice as in panel B. Bar = 50 μm. n = 6 mice/group, Student's t-test.

    Techniques Used: Staining, Knock-Out, Ligation, Over Expression

    Re-establishing redox balance suppresses sEH mediated TGFβ activation and atherosclerosis development. A. Volcano plot showing differentially regulated transcripts in endothelial cells isolated from wild type or sEH overexpressing mice. n = 3 independent cell batches/group. B. Transcriptional factor activity prediction in samples as in panel A. C. Representative immunoblot showing protein levels in human endothelial cells infected with a control (CTL) or a lentivirus overexpressing sEH and treated with solvent or N-acetyl cysteine (NAC, 1 mmol/L, 24 h). n = 5 independent cell batches/group, two-way ANOVA. D. Relative mRNA levles of TGFβ and its targets in samples as in panel C. n = 4 independent cell batches/group. E. Representative MitoSOX fluorescence in cells as in panel C. similar observations were made in 5 more independent cell batches/group F. Relative levels of MitoSOX fluorescent intensity following flow cytometry in cells as in panel C. n = 6 independent cell batches/group, two-way ANOVA. G. Representative brightfield image and oil-red-o staining of left carotid artery from endothelial cell specific sEH inducible overexpression mice (OE) treated with solvent (Sol) or N-acetyl cysteine (NAC), following 4 weeks of AAV-PCSK9, 4 weeks of HFD and 3 weeks of partial carotid ligation. Quantification of plaque area is shown on the right. Bar = 1 mm and 200 μm in brightfield image and sections, respectively. n = 4 mice/group, two-way ANOVA. H. Representative immunofluorescent image of pSMAD2 (red), CD31 (cyan) and DAPI (green) on left carotid artery in mice as in panel G. Quantification of pSMAD2 levels in endothelial cells is shown on the right. Bar = 20 μm. n = 4–5 mice/group, one-way ANOVA.
    Figure Legend Snippet: Re-establishing redox balance suppresses sEH mediated TGFβ activation and atherosclerosis development. A. Volcano plot showing differentially regulated transcripts in endothelial cells isolated from wild type or sEH overexpressing mice. n = 3 independent cell batches/group. B. Transcriptional factor activity prediction in samples as in panel A. C. Representative immunoblot showing protein levels in human endothelial cells infected with a control (CTL) or a lentivirus overexpressing sEH and treated with solvent or N-acetyl cysteine (NAC, 1 mmol/L, 24 h). n = 5 independent cell batches/group, two-way ANOVA. D. Relative mRNA levles of TGFβ and its targets in samples as in panel C. n = 4 independent cell batches/group. E. Representative MitoSOX fluorescence in cells as in panel C. similar observations were made in 5 more independent cell batches/group F. Relative levels of MitoSOX fluorescent intensity following flow cytometry in cells as in panel C. n = 6 independent cell batches/group, two-way ANOVA. G. Representative brightfield image and oil-red-o staining of left carotid artery from endothelial cell specific sEH inducible overexpression mice (OE) treated with solvent (Sol) or N-acetyl cysteine (NAC), following 4 weeks of AAV-PCSK9, 4 weeks of HFD and 3 weeks of partial carotid ligation. Quantification of plaque area is shown on the right. Bar = 1 mm and 200 μm in brightfield image and sections, respectively. n = 4 mice/group, two-way ANOVA. H. Representative immunofluorescent image of pSMAD2 (red), CD31 (cyan) and DAPI (green) on left carotid artery in mice as in panel G. Quantification of pSMAD2 levels in endothelial cells is shown on the right. Bar = 20 μm. n = 4–5 mice/group, one-way ANOVA.

    Techniques Used: Activation Assay, Isolation, Activity Assay, Western Blot, Infection, Control, Solvent, Fluorescence, Flow Cytometry, Staining, Over Expression, Ligation



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    seh overexpression mice  (Shanghai Model Organisms Center)
    90
    Shanghai Model Organisms Center seh overexpression mice
    Endothelial soluble epoxide hydrolase accelerates atherosclerosis development. A. Representative brightfield image and oil-red-o staining of left carotid artery from wild type and inducible endothelial-specific sEH knockout mice (iΔEC) following 4 weeks of AAV-PCSK9, 4 weeks of HFD and 3 weeks of partial carotid ligation. Quantification of plaque area is shown on the right. Bar = 1 mm and 200 μm in brightfield image and sections, respectively. n = 6 mice/group, two-way ANOVA. B. Representative brightfield image and oil-red-o staining of left carotid artery from wild type and inducible endothelial-specific sEH <t>overexpression</t> mice (iOE) following 4 weeks of AAV-PCSK9, 4 weeks of HFD and 3 weeks of partial carotid ligation. Quantification of plaque area is shown on the right. Bar = 1 mm and 200 μm in brightfield image and sections, respectively. n = 6 mice/group, two-way ANOVA. C. Representative immunefluorescent image of VCAM-1 (green), CD31 (red) and nuclei (DAPI) from left carotid sections of mice as in panel B. Bar = 50 μm. n = 6 mice/group, Student's t-test. D. Representative immunofluorescent image of F4/80 (green), CD31 (red) and nuclei (DAPI) from left carotid sections of mice as in panel B. Bar = 50 μm. n = 6 mice/group, Student's t-test.
    Seh Overexpression Mice, supplied by Shanghai Model Organisms Center, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/seh+overexpression+mice/pmc12214128-163-8-17?v=Shanghai+Model+Organisms+Center
    Average 90 stars, based on 1 article reviews
    seh overexpression mice - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Endothelial soluble epoxide hydrolase accelerates atherosclerosis development. A. Representative brightfield image and oil-red-o staining of left carotid artery from wild type and inducible endothelial-specific sEH knockout mice (iΔEC) following 4 weeks of AAV-PCSK9, 4 weeks of HFD and 3 weeks of partial carotid ligation. Quantification of plaque area is shown on the right. Bar = 1 mm and 200 μm in brightfield image and sections, respectively. n = 6 mice/group, two-way ANOVA. B. Representative brightfield image and oil-red-o staining of left carotid artery from wild type and inducible endothelial-specific sEH overexpression mice (iOE) following 4 weeks of AAV-PCSK9, 4 weeks of HFD and 3 weeks of partial carotid ligation. Quantification of plaque area is shown on the right. Bar = 1 mm and 200 μm in brightfield image and sections, respectively. n = 6 mice/group, two-way ANOVA. C. Representative immunefluorescent image of VCAM-1 (green), CD31 (red) and nuclei (DAPI) from left carotid sections of mice as in panel B. Bar = 50 μm. n = 6 mice/group, Student's t-test. D. Representative immunofluorescent image of F4/80 (green), CD31 (red) and nuclei (DAPI) from left carotid sections of mice as in panel B. Bar = 50 μm. n = 6 mice/group, Student's t-test.

    Journal: Redox Biology

    Article Title: Endothelial soluble epoxide hydrolase links polyunsaturated fatty acid metabolism to oxidative stress and atherosclerosis progression

    doi: 10.1016/j.redox.2025.103730

    Figure Lengend Snippet: Endothelial soluble epoxide hydrolase accelerates atherosclerosis development. A. Representative brightfield image and oil-red-o staining of left carotid artery from wild type and inducible endothelial-specific sEH knockout mice (iΔEC) following 4 weeks of AAV-PCSK9, 4 weeks of HFD and 3 weeks of partial carotid ligation. Quantification of plaque area is shown on the right. Bar = 1 mm and 200 μm in brightfield image and sections, respectively. n = 6 mice/group, two-way ANOVA. B. Representative brightfield image and oil-red-o staining of left carotid artery from wild type and inducible endothelial-specific sEH overexpression mice (iOE) following 4 weeks of AAV-PCSK9, 4 weeks of HFD and 3 weeks of partial carotid ligation. Quantification of plaque area is shown on the right. Bar = 1 mm and 200 μm in brightfield image and sections, respectively. n = 6 mice/group, two-way ANOVA. C. Representative immunefluorescent image of VCAM-1 (green), CD31 (red) and nuclei (DAPI) from left carotid sections of mice as in panel B. Bar = 50 μm. n = 6 mice/group, Student's t-test. D. Representative immunofluorescent image of F4/80 (green), CD31 (red) and nuclei (DAPI) from left carotid sections of mice as in panel B. Bar = 50 μm. n = 6 mice/group, Student's t-test.

    Article Snippet: sEH-tdTomato reporter mice ( Ephx2 LSL-tdTomato ) and sEH overexpression ( Rosa26 CAG-LSL-Ephx2−3xFlag ) were generated by Shanghai Model Organisms Center (Shanghai, China) using CRISPR/Cas9 system in a C57BL/6J mouse background.

    Techniques: Staining, Knock-Out, Ligation, Over Expression

    Re-establishing redox balance suppresses sEH mediated TGFβ activation and atherosclerosis development. A. Volcano plot showing differentially regulated transcripts in endothelial cells isolated from wild type or sEH overexpressing mice. n = 3 independent cell batches/group. B. Transcriptional factor activity prediction in samples as in panel A. C. Representative immunoblot showing protein levels in human endothelial cells infected with a control (CTL) or a lentivirus overexpressing sEH and treated with solvent or N-acetyl cysteine (NAC, 1 mmol/L, 24 h). n = 5 independent cell batches/group, two-way ANOVA. D. Relative mRNA levles of TGFβ and its targets in samples as in panel C. n = 4 independent cell batches/group. E. Representative MitoSOX fluorescence in cells as in panel C. similar observations were made in 5 more independent cell batches/group F. Relative levels of MitoSOX fluorescent intensity following flow cytometry in cells as in panel C. n = 6 independent cell batches/group, two-way ANOVA. G. Representative brightfield image and oil-red-o staining of left carotid artery from endothelial cell specific sEH inducible overexpression mice (OE) treated with solvent (Sol) or N-acetyl cysteine (NAC), following 4 weeks of AAV-PCSK9, 4 weeks of HFD and 3 weeks of partial carotid ligation. Quantification of plaque area is shown on the right. Bar = 1 mm and 200 μm in brightfield image and sections, respectively. n = 4 mice/group, two-way ANOVA. H. Representative immunofluorescent image of pSMAD2 (red), CD31 (cyan) and DAPI (green) on left carotid artery in mice as in panel G. Quantification of pSMAD2 levels in endothelial cells is shown on the right. Bar = 20 μm. n = 4–5 mice/group, one-way ANOVA.

    Journal: Redox Biology

    Article Title: Endothelial soluble epoxide hydrolase links polyunsaturated fatty acid metabolism to oxidative stress and atherosclerosis progression

    doi: 10.1016/j.redox.2025.103730

    Figure Lengend Snippet: Re-establishing redox balance suppresses sEH mediated TGFβ activation and atherosclerosis development. A. Volcano plot showing differentially regulated transcripts in endothelial cells isolated from wild type or sEH overexpressing mice. n = 3 independent cell batches/group. B. Transcriptional factor activity prediction in samples as in panel A. C. Representative immunoblot showing protein levels in human endothelial cells infected with a control (CTL) or a lentivirus overexpressing sEH and treated with solvent or N-acetyl cysteine (NAC, 1 mmol/L, 24 h). n = 5 independent cell batches/group, two-way ANOVA. D. Relative mRNA levles of TGFβ and its targets in samples as in panel C. n = 4 independent cell batches/group. E. Representative MitoSOX fluorescence in cells as in panel C. similar observations were made in 5 more independent cell batches/group F. Relative levels of MitoSOX fluorescent intensity following flow cytometry in cells as in panel C. n = 6 independent cell batches/group, two-way ANOVA. G. Representative brightfield image and oil-red-o staining of left carotid artery from endothelial cell specific sEH inducible overexpression mice (OE) treated with solvent (Sol) or N-acetyl cysteine (NAC), following 4 weeks of AAV-PCSK9, 4 weeks of HFD and 3 weeks of partial carotid ligation. Quantification of plaque area is shown on the right. Bar = 1 mm and 200 μm in brightfield image and sections, respectively. n = 4 mice/group, two-way ANOVA. H. Representative immunofluorescent image of pSMAD2 (red), CD31 (cyan) and DAPI (green) on left carotid artery in mice as in panel G. Quantification of pSMAD2 levels in endothelial cells is shown on the right. Bar = 20 μm. n = 4–5 mice/group, one-way ANOVA.

    Article Snippet: sEH-tdTomato reporter mice ( Ephx2 LSL-tdTomato ) and sEH overexpression ( Rosa26 CAG-LSL-Ephx2−3xFlag ) were generated by Shanghai Model Organisms Center (Shanghai, China) using CRISPR/Cas9 system in a C57BL/6J mouse background.

    Techniques: Activation Assay, Isolation, Activity Assay, Western Blot, Infection, Control, Solvent, Fluorescence, Flow Cytometry, Staining, Over Expression, Ligation